400 Farmington Avenue, Cell & Genome Sciences Building, Imaging Suite R1510 |
(Location: R1515) |
The 510 is mounted on an Axiovert 100M, with automated xyz control. Lasers include an argon laser with emission at 458, 488, and 514nm, and two HeNe lasers with emission wavelengths at 543 and 633 nm. The system has 3 photomultiplier tubes and a transmitted light detector. |
(Location: R1521) |
The CC3 is a combined confocal and FCS system, mounted on a AxioVert 200M. This system has two avalanche photodiodes (APD) with ultimate sensitivity allowing for decreased laser power with delicate specimens as well as three traditional PMTs. The APD's are highly efficient in the red wavelength range where fluorescent signal can be very weak.This system is equipped with the following laser lines: argon 458, 477, 488, 514nm, DPSS 561nm, and HeNe 633nm. |
(Location: R1523) |
Combined confocal/FCS/NLO system, mounted on an inverted Axio Observer Z1. The 780 employs the spectral 32-channel QUASAR GaAsp detector allowing for spectral imaging, maximum sensitivity photon counting and Fluorescence Correlation Spectroscopy (FCS) as well as two adjacent PMT's. This system is equiped with the following laser lines: 405 and 440, 458, 488, and 514, 561, 633. Multi-photon excitation is available from 720 - 950 nm. |
(Location: R1518) |
Spinning Disk Confocal on an inverted, Nikon TE2000 microscope capable of exciting with the following laser lines: 442, 488, 514, 568 and 647 nm. This system employs the Micropoint Mosaic system for photomanipulation techniques such as FRAP or Photoactivation. |
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Widefield Microscopes |
(Location: R1522) |
Upright widefield microscope, equipped with a Photometrics PXL-EEV37 high speed digital cooled CCD camera. Molecular Devices MetaMorph, for image acquisition, analysis and image processing. Basic filter sets include Rhodamine, FITC and DAPI. Equipped for DIC and phase contrast. |
(Location: R1514) |
The TIRF system is equipped with the Zeiss Axio Observer (inverted) )microscope and the Zeiss AxioCam MRm, a 12 bit, high sensitivity, optimum resolution camera. The fluorescent system captures a thin optical section of approximately 100 -200 nm thick which allows for the visualization of processes taking place near the cell membrane of living cells.This system employs the α-Plan-Fluar 100x/1.45 oil objective. |
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User Computers |
(Location: R1510 Imaging Suite) |
64 bit computer with ImarisSurpass 3-D rendering and analysis software, MetaMorph, Photoshop, and Zeiss LSM and Zen Image Browsers. |
(Location: R1510 Imaging Suite) |
PC equipped with MetaMorph, Photoshop, ImageJ, Zeiss LSM and Zen Image Browsers and a Flatbed Scanner. |
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Other |
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CCAM microscope objective,( if you need only the objective and no microscope) and heater signup |
